Ethidium bromide is commonly used to detect nucleic acids in molecular biology laboratories. In the case of DNA this is usually double-stranded DNA from PCRs, restriction digests, etc. Single-stranded RNA can also be detected, since it usually folds back onto itself and thus provides local base pairing for the dye to intercalate.

Produktnamn. OmniPur® Ethidium Bromide Solution 10 mg/ml. Säkerhetsdatabladen för katalogartiklar finnstillgängliga på www.merckgroup.com. Sida 2 av 17.

Introduction. Ethidium bromide intercalates (inserts itself) between nucleic acid base pairs and exhibits fluorescence under ultraviolet light, providing molecular biologists with an excellent stain for visualizing and imaging nucleic acids. BTW, Ethidium bromide is found to be toxic against yeast, single cells, and unprotected embryos. There is no direct evidence of its mutagenicity or genotoxicity against animals or humans, not in Ethidium Bromide is classified as hazardous chemical waste Solutions should be deactivated or filtered.

Ethidium bromide

Additionally, it can absorb energy from nucleotides excited by absorbance of 260 nm radiation. Ethidium re-emits this energy as yellow/orange light centered at 590 nm. The fluorescence of ethidium bromide in aqueous solution is significantly lower than that of the interchelated You're visiting the Staff Portal without being logged in. If you login you will get access to personalized content. Log in External users Ethidium Bromide AR 1239-45-8 100 Toxicological Data on Ingredients: Not applicable. Potential Acute Health Effects: Hazardous in case of ingestion. Slightly hazardous in case of skin contact (irritant), of eye contact (irritant), of inhalation.

poly(dT) is characterized using linear dichroism and circular dichroism spectroscopy, and thermal denaturation. The two monomeric dyes ethidium bromide and

Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide. Ethidium bromide is a sensitive fluorescent stain for visualizing DNA in agarose and polyacrylamide gels. It is excited with a standard 302 nm UV transilluminator and emits a red-orange signal that can be photographed with Polaroid film or with a CCD-based gel documentation system.

Ethidium bromide is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis. It fluoresces readily with a reddish-brown color when exposed to ultraviolet light, intensifying almost 20-fold after binding to DNA.

Despite extensive studies of ethidium bromide complexes with polynucleotides, a satisfactory mechanism explaining the high degree of fluorescence enhancement upon binding has not A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide. 2007-09-26 Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

Ethidium bromide, commonly employed in molecular biology laboratories, has been widely used for many years as a nucleic acid stain and a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis. Ethidium bromide possesses UV absorbance maxima at 300 and 360 nm. Additionally, it can absorb energy from nucleotides excited by absorbance of 260 nm radiation. Ethidium re-emits this energy as yellow/orange light centered at 590 nm. The fluorescence of ethidium bromide in aqueous solution is significantly lower than that of the interchelated You're visiting the Staff Portal without being logged in.
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CAS: 1293-45-8. 5% Ethidium Bromide.

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Ethidium bromide is used to visualize DNA in agarose gels and in many cesium chloride gra- dient protocols. The 10 mg/ml ethidium bromide stock solution can be

A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide.